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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70113-262-792
中文名称:
英文名称:2-3/4 SQUARE X 2 HIGH BOX
货期:热销品现货,其他请咨询




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文献和实验HIGH EFFICIENCY TRANSFORMATION
for a few minutes. The grid area is typically 1 square millimeter, divided into 25 equal-sized squares, and the volume measured is 10-4 ml. ii) Count the number of cells in 5 diagonal squares iv) Calculate the cell titer as follows: cells counted x 5 x
High Efficiency Yeast Transformation (Gietz Long Protocol)
.1. Inoculate 2-5 mls of liquid YPAD or 10 ml SC and incubate with shaking overnight at 30℃.2. Count o/n culture and inoculate 50 mls of warm YPAD to a cell density of 5 x 106/ml culture.Note:i) Dilute overnight YPAD or SC cultures 10-1 or more in water.ii
High efficiency LiAc transformation
High efficiency LiAc transformation Modified from Gietz Homepage. Stuff you need: 10X LiAc 1 M LiAc, filter sterilize 50% PEG 3350 , filter sterilize 1. Inoculate 2-5 mls of liquid YPED or 10 ml synthetic
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