赛默飞世尔Thermo Fisher CLEAR POLY

Tg 008 Southern Blotting

Paper GB002 (Schleicher %26amp; Schuell, 34550) Gel Blot Paper GB004 (Schleicher %26amp; Schuell, 34340) Whatman paper, 3MM Contrad 70 detergent (Fisher, 04-355-1) 8 x 10 film cassette (Fisher, FB-XC-810) 14 x 17 film cassette (Fisher, FB-XC-1417 8 x 10

Preparation of Nested Deletions

. Prehybridization Sol'n Hybridization Sol'n ddH2O 15.3 ml ddH2O 15.3 ml Formamide 15 ml Formamide 15 ml 20 x SSPE 12.5 ml 20 x SSPE 12.5 ml 20% SDS 0.25 ml 20% SDS 0.25 ml 50 x Denhardt's 5 ml 50 x Denhardt's 5 ml Salmon Sp. DNA 2 ml Salmon Sp. DNA 2 ml Poly A 5 µl

RNA实验方法

?base0.5 mM50 mM18.61 gNa2 EDTA5 mM500 mM41.04 gNaOAc-anhydrous~43 mLGlacial acetic acid to pH 7.8x mLType I water1000 mL100x TAE5 is composed of 1 M Tris base, 50 mM Na2 EDTA, 500 mM NaOAc and approximately 43 mL of glacial acetic acid (to pH