赛默飞世尔Thermo Fisher GLASS BURET

原创：western protocol

-Cl, pH8.0 1.2114 20% (v/v) Glycerol 20g 4% (w/v) SDS 4g 0.2% Bromophenol blue 0.2g 200mM DTT 20ul 5x Running buffer: 1 L 15.1 g Trizma base (= 125m M) 94g Glycine (= 1.25 M) 50ml 10%SDS (= 0.5%)--add last Do not adjust the pH!! 10x Blotting

Mouse Compete Blood Counts

of the total.   Red cell count: Dilute cells 1/1000 in PBS. Add 10 µL to a hemocytometer. Count the number of RBC per large square. Calculate: RBC/large square x 1,000 dilution x 10 large squares/µL = RBC/µL blood.   White cell count: Add 10 µL

DNA Sequencing

, 150mM dATP, 150mM dCTP, 150mM c7deaza-dGTP and 15mM of the respective ddNTP) 5 x DNA labelling mix (10mM dGTP, 10mM dCTP, 10mM dTTP, 200mM Tris.Cl, pH 7.5, 250mM NaCl) 300mM DTT [a-35S]-dATP (~ 1000Ci/mmol, Amersham or DuPont) T7 DNA polymerase