链脲佐菌素(STZ),Streptozotocin

 

SKU	0210055701
Alternate Names	N-(Methylnitrosocarbamoyl)-α-D-glucosamine; Streptozocin; STZ; 2-Deoxy-2-(3-methyl-3-nitrosoureido)-D-glucopyranose
Application Notes	链脲佐菌素（Streptozotocin, STZ）是一种由链球菌产生的葡萄糖-亚硝脲，含N-亚硝基化合物。链脲佐菌素用于诱导糖尿病的医学研究。STZ对胰岛β细胞具有特异性毒性，可以用来诱导建立糖尿病动物模型及治疗胰岛β细胞瘤。相比于其他诱导糖尿病的方法，STZ诱导建立的糖尿病模型对机体组织毒性小，动物存活率高，是目前国内外糖尿病造模使用最为广泛的一种方法。
Base Catalog Number	100557
Beilstein Registry Number	2060675
Biochemical Physiological Actions	An N-nitroso-containing compound that acts as a nitric oxide donor in pancreatic islets; induces death of insulin-secreting cells, producing an animal model of diabetes. Potent DNA methylating agent that induces chromosomal breakage. Cytotoxic to neuroendocrine tumor cell lines that express the GLUT2 glucose transporter.
CAS #	18883-66-4
EC Number	242-646-8
Extinction Coefficient	EmM(228 nm): 6.36 (ethanol)
Format	Powder
Half Life	biological half-life in cell culture medium was shown to be approximately 19 minutes.
Hazard Statements	H350
Melting Point	239° F (Decomposes) (NTP, 1992)
Molecular Formula	C8H15N3O7
Molecular Weight	265.222 g/mol
Personal Protective Equipment	Eyeshields, full-face particle respirator type N100, Gloves, respirator cartridge, respirator filter
Purity	~75% α-anomer basis; ≥97%
RTECS Number	LZ5775000
Safety Symbol	GHS06+GHS08
Solubility	Soluble (NTP, 1992)
Typical Working Concentration	Has been used in intravenous injections in rats at a dose of 65 mg/kg body weight to induce diabetes (using cold 0.1 M citrate buffer pH 4.5). In rats and dogs, diabetes was induced using intravenous dosage of 50 mg/kg body weight (using 1-2% w/v solutions in saline buffered with citrate dextrose solution at pH 5.0).
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

STZ诱导I型糖尿病

方法：         I型糖尿病显著的病理生理学特征为胰岛β细胞数量显著减少和消失所导致的胰岛素分泌显著下降或缺失。采用较大剂量一次性注射的给药方法，可直接引起胰岛β细胞的广泛破坏，造成Ⅰ型糖尿病模型。

优势：         方法简便，成模时间短，成模率高，与人类I型糖尿病表现非常相似。

高脂饲料+STZ诱导Ⅱ型糖尿

方法：         Ⅱ型糖尿病显著的病理生理学特征为胰岛素调控葡萄糖代谢能力的下降（胰岛素抵抗）伴随胰岛β细胞功能缺陷所导致的胰岛素分泌减少（或相对减少）采用高糖高脂饲养并配合低剂量注射的方法，可建立Ⅱ型糖尿模型。胰岛素抵抗可以通过高糖、高脂饮食实现。而胰岛细胞功能缺陷引起的胰岛素分泌减少一般采用STZ诱导。

优势：         实验周期短、方法简便、相对可靠稳定、造模成本低、且诱导的Ⅱ型糖尿病模型症状和发病机制与人类Ⅱ型糖尿病非常相似。

Application Notes

链脲佐菌素用于诱导糖尿病的医学研究。