赛默飞世尔Thermo Fisher Bottle brus

Procedures for In Vitro Production of Bovine Embryos

(this should have been done beforehand) 2) fill bottle/beaker with some dilute PCC and shake 3) clean with brush 4) rinse 5X with warm/hot tap water (make sure there is no soap left) 5) rinse 5X with deionized water 6) rinse 1X with distilled/deionized water 7) Let air dryPlastic beakers/containers

In Situ HYBRIDIZATION TO TISSUE SECTIONS

. Remove the chuck when the O.C.T. starts to turn white but before it's completely frozen. Place a second, smaller, drop of O.C.T. on top of the unfrozen portion of the first. Quickly place the embryos in the center of the drop

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

of 20 mM HEPES Buffer in 0.9% saline solution. In a separate conical tube, create a T solution by adding 17 μl of the thrombin stock, and 1.3 μl of 2 N Ca solution to 135 μl of DMEM. See Table 1.    2)      In a third conical tube, prepare