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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
peptides, cytokines and apoptosis in lower and higher vertebrates. J. Neuroimmunol . 72: 67. 4. Ueda, N., Shah, S.V. 1994. Apoptosis. J. Lab. Clin. Med . 124: 69. B) TUNEL in situ procedure B.2.1 Materials proteinase K (pK) (A2
Cryopreservation of cell cultures
). 2. Remove the growth medium and wash twice in PBS (B0.1 ml cm2). 3. Add prewarmed (37 1C) trypsin solution (0.5 ml per 25 cm2) to cover the cell monolayer, recap the flask and incubate atB37 1C for 5 min. 4. Examine the monolayer and tap
to the bottom, top and middle of the sample tube. If the density and viscosity of the medium are known, as well as the density of a given particle, then the time needed to completely sediement a particle can be determined by the formula: T = ((D-L)/(D+L))*(N/(d(g-p)S
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