赛默飞世尔Thermo Fisher PELLET FEED

Procedures for In Vitro Production of Bovine Embryos

over a sterile 100 ml beaker. Using a plastic pasteur pipet and sterile technique, aspirate the pellet of oocytes at the bottom of each tube (figure 8C). Slowly pour the aspirated pellet into the cell strainer (figure 8D). Up to 3 tubes of oocytes

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v

原代神经细胞培养方法 Neuron Cell Culture

. Place all 4 ml into a 15 ml centrifuge tube and spin for about 8 min . Discard the supernatant and add 2 ml of IMDM to the pellet . Disperse the cells by repeatedly aspiring them with a 5ml pipette