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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
人滋养细胞；HTR-8
- 库存：
10
- 组织来源：
上皮样
- 生长状态：
贴壁生长
- 规格：
T25
生长特征：贴壁生长
培养条件:DMEM/F12+15%FBS
传代方法:1：2传代
冻存条件：无血清细胞冻存液
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文献和实验Using HTR Reagent to Clean Up DNA Samples with PCR Inhibitors
3. Centrifuge at full speed (>13,000 x g) for 2 minutes. 4. Transfer cleared supernatant to a new 1.5 ml tube. Note: If the supernatant still shows dark color from soil at this point, perform the HTR extraction again by repeating step 1-4. 5. Add
Using Soil DNA Kit (D5625) to Clean Up DNA containing inhibitors
3. Centrifuge at full speed (>13,000 x g) for 2 minutes. 4. Transfer cleared supernatant to a new 1.5 ml tube. Note: If the supernatant still shows dark color from soil at this point, perform the HTR extraction again by repeating step 1-4.
Stool DNA Protocol (for pathogen detection)
for 2 minutes. 11. Centrifuge at full speed (>13,000 x g) for 2 minutes to pellet the inhibitors absorb to HTR Reagent. 12. Transfer 250 ul supernatant to a new 2.0 ml tube. 13. Optional: If RNA-free DNA is required, add 10 ul RNase A and mix
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