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货号:T_70103-375-3KK
中文名称:Target LoVial 直口压盖瓶
英文名称:VIAL TARGET GREEN BAND 100/PK
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文献和实验Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
(TU)/mL and is calculated by the following formula: Titer = % GFP-positive cells (number of cells at the time of infection/100) x dilution Multiplicity of infection (moi) is the ratio of infectious particles to target cells
Protocol for competitive RT-PCR
of each dNTP, 250 nM of each primer and 1/10 volume of a 10x PCR standard buffer (15 mM MgCl2; 100 mM Tris/HCl, pH 8.3; 500 mM KCl) are added to a total volume of 50 µl. The PCR is run in the thermal cycler GeneAmp 9600 (Perkin Elmer). The PCR products
START" PCR.NOTE:USE PLUGGED PIPETTE TIPS: prevents aerosol contamination of pipettes.Use of detergents is recommended only for Taq from Promega (up to 0.1% v/v, Triton X-100 or Tween-20). DMSO apparently allows better denaturation of longer target
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