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999
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北京泽平
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热销品大量现货,其余请咨询
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货号:T_70311888601
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英文名称:PEN BLUE
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文献和实验substitute (Fisher). - 100% ethanol, 95% ethanol and 80% ethanol. Prepare the 80% ethanol fresh each day. - Phosphate buffered saline (PBS) pH 7.4 - Oxidation blocking solution (PBS + 2% hydrogen peroxide). Make fresh
Clone Genes From a Phage Library
nylon filters from Fisher (MSI N00HY08250, 82 mm, 50/box). These are quite cheap. Solutions: LB, SM solution, Church hybridization solution, Church wash solution, Chloroform (see the end for recipes).Ex-assist phage as well for plasmid excision
for several weeks.Use 50 microliters per plate. Filters: I like simple uncharged nylon filters from Fisher (MSI N00HY08250, 82 mm, 50/box). These are quite cheap. Solutions: LB, SM solution, Church hybridization solution, Church wash solution, Chloroform
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