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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
1年
- 规格:
EA
货号:T_70315118354
中文名称:
英文名称:X10 lid clip for Eppendorf tube 5ml
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文献和实验Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels
out of the gel box, carefully remove one dialysis clip, and pipet the liquid out of the bag into a sterile Eppendorf tube. A Pasteur pipet works well for this pipetting. 9-Rinse the inside of the bag and gel slice with TE and combine this rinse
Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels
in half again, lengthwise. 3 -Place two slices (which together would make up one half-plug) inside a sterile Eppendorf tube containing 900 µl sterile 10 mM Tris-HCl (pH 7.5). The pieces should fit into the bottom of the tube. 4 -Place
can be increased to 75 mM in the Elution buffer if final DNA yields are consistently low). 5. Add 0.6 ml of Elution buffer and collect the flow-through in sterile eppendorf tubes placed in a Sarstedt rack. Transfer a 10 µl aliquot into another eppendorf tube
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