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999
- 供应商:
北京泽平
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1年
- 规格:
EA
货号:T_70103-378-390
中文名称:National 样品瓶内管
英文名称:INSERT SHELL 0.75ML 100/PK
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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
7.0 µl 10x Taq Buffer 2.5 µl 25 mM MgCl2 1.5 µl 10 x dNTP mix 5.0 µl Primer * (30 ng/µl) 5.0 µl Digested DNA (100-200 µg/ml) 2.0 µl mineral oil 40 µl * For cDNAs cloned in Bluscript, we use the following primers (21mers): "T
cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick) Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel: Add 678 μl
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
(100 units/ml and 100 μg/ml respectively) and 1.98 g of glucose to 250 ml 1x sterile phosphate buffered saline (PBS) and bring solution volume to 500 ml with additional sterile 1x PBS. 2) Prepare stop media by adding 25 ml FBS and 5 ml
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