赛默飞世尔Thermo Fisher DRYING TUBE

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Expression Library Screening (Procaryotic) Using AP-Fusion Proteins

in 37°C shaker incubator (usually 6-8h) Add 4.5 ml of a sterile glycerol-media solution (5ml of autoclaved 80% glycerol solution + 4ml LB + 9µl 1000x tetracyclin stock). Mix well. Aliquot into sterile centrifuge tubes (1ml/tube

In Situ HYBRIDIZATION TO TISSUE SECTIONS

the nitrogen touch the O.C.T.). Put the chuck with embryos in the cryostat so they can come up to temperature. Cutting frozen sections Take the chuck (plus embryos!) out of the cryostat (they should be at cryostat tempera- ture, -14 to -19