赛默飞世尔Thermo Fisher X6 VOL PIPE

Karyotyping of X. tropicalis

for 20 minutes. 5. Pipette the dorsal halves, carrying as little water with them as possible, into an Eppendorf tube containing 0.2 ml of 60% acetic acid in water. 6. Allow to stand for 5 minutes. 7. Pipette all of the tissue, carrying

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

with 0.5x MBS at RT. (5)   Transfer tissues into a 1.5 ml microfuge tube using a wide-bore plastic pipette. Remove the MBS as much as possible. (6)  You may store the tissues at �80°C (use liquid nitrogen to freeze them). (7)  Add 0.5 ml of �

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

    Dissecting microscope;       Acid Tyrode's (e.g. Sigma: T1788);       Sterile plastic Petri dishes (100 x 15 mm);       M2 and KSOM medium in 1 ml syringes;       9'' Pasteur pipettes;       Mouth or Finger controlled pipette. Method: Place