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- 文献和实验
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999
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北京泽平
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EA
货号:T_70311844120
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英文名称:X2 VOLUMETRIC FLASK 20ML NS12/21
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文献和实验Procedures for In Vitro Production of Bovine Embryos
all debris behind (repeat twice to assure that oocytes are clean of debris; Figure 10).Figure 10. An X-plate used to wash COCs, purchased from Fisher .Figure 11. Washing of COCs using an X-plate.Figure 12. Some instruments used to pick up embryos
Preparation of Embryonic Extract
. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml
Human FastTrack mRNA Isolation
Oligo(dT) Cellulose 1.Resuspend oligo(dT) in 20ml Binding Buffer by vortexing. 2.Centrifuge at 3,000 x g for 5 minutes at room temperature. 3.Resuspend oligo(dT) in 10ml Binding Buffer by vortexing. 4.Centrifuge at 3,000 x g for 5 minutes at room
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