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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70103-410-503
中文名称:
英文名称:BOX VIAL CRYO-SAFE 2ML 24CS
货期:热销品现货,其他请咨询




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文献和实验Freezing and Thawing of Mammalian Cell Lines
10e6 cells/0.5 mL freezing medium. Aliquot in cryo-vials 0.5 mL/vial. Place vials upright in a styrofoam box and cover well. Place in a -70 °C for 24 hours. Place vials in a wand and put in a liquid nitrogen container. Thawing
Freezing and Thawing of Mammalian Cell Lines
% FCS, 70% media that you used to grow the cells such as RPMI or DMEM) at a concentration of 2 X 10e6 cells/0.5 mL freezing medium. Aliquot in cryo-vials 0.5 mL/vial. Place vials upright in a styrofoam box and cover well. Place in a -70 °C for 24
ES Cell Culture and Manipulation
tips (preferably barrier, or plugged tips), a sterile 96well round-bottom plate, plenty of ES cell media, and an inverted microscope in a hood. Aspirate the REF media from the 24-wells and add 2ml of ES cell media to each well. Add 40 µl trypsin-EDTA
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