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货号：T_7015868-16
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英文名称：KHP, 1000 PPM COD, 500 ML
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration
Procedures for In Vitro Production of Bovine Embryos
). Prepare 10 ml aliquots of BSS in sterile 13 ml tubes and store at -20°C indefinitely.Stock 4: BSS/Hep. Add 1000 USP units of sterile heparin (dissolved in 3-5 ml of water and sterilize through a syringe filter) into 500 ml BSS (Stock 3). Store
, which is enough for two 0.8 mm thick gels, or one 1.5 mm gel.5% 6% 7% 8% 9% 10%5 6 7 8 9 10 ml 30% acrylamide/bis7.5 7.5 7.5 7.5 7.5 7.5 ml 1.5 M Tris 8.817 16 15 14 13 12 ml H2OThen add
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