赛默飞世尔Thermo Fisher PVC TRAY LO

Immobilized pH gradient (IPG) as first dimension

in a form to allow sample application in sample cups of 16 x 11 x 6 mm [8].   IPG gel strips rehydration Hydration was performed overnight in the Pharmacia reswelling cassette with 25 ml of a solution containing urea (8 M), CHAPS (2% w/v), DTE (10

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

and cathode buffers (see reciperecipes ) Electrophoresis apparatus: Protean II 16‐cm cell (Bio‐Rad) or SE 600 16‐cm unit (Hoefer) with clamps, glass plates, casting unit, and buffer chambers

小RNA的Northern BLOT

. Rinse with distilled water; double distilled water and ethanol.  Put 1.5 mm thick spacers on the 20 x 40 cm glass plate, put the notched glass plate on top of the spacers. Clamp the plates together on one side, top and bottom with large binder