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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70114-559-464
中文名称:
英文名称:BIOHIT TIP 10 UL PK1000
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
. Do one case at a time. If normal is being dissected do this first. Scrape away areas that you dont want away from the sample (use a #11 blade). Take a new #15 blade and dip (don't dunk) tip of blade into the labeled tube with pcr pk
RUVKUN Antibody Staining Protocol revised 10/29/90
Triton buffer + 1% BME, 37 C, more than 2 hours with agitation. (Some think BME step isn't necessary.) After this point the worms are fragile and shouldn't be spun hard: approximately 1000 rpm. Wash worms once in 1X BO3 buffer. 100X BO3 buffer ( pH 9.2
loading tips 吸。 7、含 beads 的 samples 离心时,有的 protocol 上推荐是 1000rpm,45 秒,但可以根据情况调整,但要注意转速不能太快防止beads 破碎。(当然如果采用的是 magnetic 的 beads 就不存在这个问题) 8、reverse crosslink 可以是 65°4 个小时,也可以 overnight。 9、reverse crosslink 后的在进行下面步骤之前建议先离心,把蒸发到离心管盖子上的部分离下来。 10、每次行 real
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