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999
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北京泽平
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货号：T_70119-123-307
中文名称：
英文名称：FOOT PLATE SS
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文献和实验(original reference: "tissue culture made easy" by Christian LaMantia from the Magnuson lab) 1) Plate cells onto gelatinized plates (35 or 60 mm) without feeders and culture 24 hours. A 1:4 split is best for most lines. 2) Culture
Large-scale yeast transformation
, 50 mls 10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar from VWR 29442-310, $24/100
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
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