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文献和实验Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
In Situ HYBRIDIZATION TO TISSUE SECTIONS
stock in TE. Phenol extract (2X) and NaOAc/EtOH ppt. to clean up. 100X Denhardts : 2% of each Bovine Serum Albumin (SIGMA A7906, Fraction V), Ficoll 400 (SIGMA F4375) and Polyvinylpyrrolidene (SIGMA PVP360). Store @ -20 degrees C. 50% (w/v
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
DNA from salmon testes (Sigma, Cat# D-1626) - Formaldehyde (37%, Fisher, Cat# F79-500)- Formamide (Boehringer Mannheim Cat# 100 731)- Glycerol (Boehringer Mannheim Cat#100 649)- Glycine (Sigma Cat# G-4392)- Glycogen (Boeringher Mannheim Cat
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