赛默飞世尔Thermo Fisher 2ML CRIMP T

Polyclonal and Monoclonal Antibodies

% ( 1000X ) Protocol: 1) ADSORBTION OF ANTIGEN a) Dilute Ag to 10 ug/ml in PBS. b) Add 100 ul of Ag solution to each well. c) Leave O.N covered with saran wrap, at 4 C. 2) BLOCKING a) Wash unbound Ag by inverting

Prime‐, Stress‐, and Cue‐Induced Reinstatement of Extinguished Drug‐Reinforced Responding in Rats: Cocaine as the Prototypical Drug of Abuse

of 0.9% sterile physiological saline 30‐ml vial of 1000 U/ml heparin solution One 10 ml vial of 100 mg/ml ketamine HCl

Stripping Western Blots

can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix