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999
- 供应商：
北京泽平
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- 保修期：
1年
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EA
货号：T_70110-200-35
中文名称：
英文名称：FLASK YELLOW SCALE 125ML 12/CS
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文献和实验Large Scale Tubulin Preparation
2. Collect homogenate (~8-9 liters), transfer into 36 GSA bottles and spin 90' at 12K in a GSA rotor at 4¡C. 3. Collect supernatant and transfer 1 liter to a 1.8L glass Fernbach flask that has 500 ml of 37¡C glycerol. Add 0.1 mM GTP, 0.5 mM ATP
SMALL Scale Transformation 1. Inoculate 1 ml of YPDA or SD with several 2�3 mm colonies. 2. Vortex vigorously to disperse any clumps. 3. Transfer cells to a flask containing 50 ml of YPDA or SD: 4. Incubate
Large Scale Tubulin Preparation
and equilibrated for use. Solutions & Supplies : 220 grams Phosphocellulose 5L 0.5N NaOH 5L 0.5N HCl 13L 0.5M K-Phosphate, pH 6.8 5L ddH2 0 5 2L beakers 12L CB + 1M KCl 20L CB 300 ml of 30 mg/ml BSA in CB
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