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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310166830
中文名称:
英文名称:Syringe,10µl,Removeable Needle for HP, 0,63mm O.D.
货期:热销品现货,其他请咨询




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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS
Grip 35 x 10 mm, Falcon 3001-3); KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410); aggregation (darning) needle ( DN-09, BLS Ltd., Hungary ,) 70% ethanol. Method: 1. Place few rows
thick gels or one 1.5 mm gel627 µl 30% acryl:bis780 µl 1M Tris pH 6.85 ml H2031 µl 20% SDS62 µl 10% AP3.8 µl TEMEDPour in between plates, or squirt in with a pasteur pipette. Carefull not to get airbubbles trapped around the teeth of the comb
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