赛默飞世尔Thermo Fisher X300 OPEN T

How to build a BAC library

in a volume of between 120 and 150µl. Reaction Mixture: 100 µl DNA 1.8 µl pBAC (20 ng/ml) 12.0 µl 10 X ligation buffer 2.0 µl 10X PA 0.5 µl ligase 400U/ul 3.7 µl H2 O Combine insert DNA, vector, PA, and H2O. Heat 5 minutes at 65°C, cool on ice. Add ligase buffer and enzyme

Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarray and Other Applicati

(Fisher Scientific) 1.5‐ml screw‐cap polypropylene centrifuge tubes 55°C and 65°C heating

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

Toluene (Acros) Methylene chloride, anhydrous (CH 2 CH 2 , Fisher) Silica gel (60 Å, 230‐400 mesh; Merck