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999
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北京泽平
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货号:T_70310217634
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英文名称:X2 PYREX GAZ DIST. TUBE DIAM15 P0
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文献和实验In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
. 10) Wash 3x in PBTw. 11) Divide gonads among several 6 x 50 mm culture tubes (Pyrex tubes are probably best as they can be baked to kill RNase), one tube per hybridzation. Hybridization 12) Place gonads in 400 ul of 50% PBTw / 50
in the stir bar. 13.Bring the vlume up t 1 liter by adding mre f the Lysis buffer. 14.Add Tritn-X100 t a final cncentratin f 2% by vlume. Nte: The Tritn-X-100 will nt disslve immediately.It will g int slutin slwly ver night. 15.Stir gently ver night
RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes
glycine / 0.1% Tween 20. 10) Wash 3x in PBTw. 11) Divide gonads among several 6 x 50 mm culture tubes (Pyrex tubes are probably best as they can be baked to kill RNase), one tube per hybridzation
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