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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
. Do not tighten excessively. 7. Add 50 μl of the cells suspended in Chemotaxis Buffer to each well on the upper chamber. To avoid creating bubbles, touch the pipette tip to the side of the chamber (don't touch the filter) and expel the cells
The Under-Agarose Migration Assay
#5 ) and resuspend the cells in migration medium at 107 cells per ml. Use Migration Medium with plates prepared with RPMI/BCS and HEPES Migration Medium with plates prepared with 2X HEPES Migration Medium. 2. Fill the two most peripheral wells with 10 μl
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