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货号:T_70310618101
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英文名称:X6 BOTTLES,WASH POLYPROPYLENE COPOLYMER 500ML (PACK OF 6)
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Large Scale Tubulin Preparation
(filtered) 1-1.5L cleaned column housing 2 stirring rods 2 aspirators with large traps Peristaltic pump 10 ml plastic pipets as inlets for peristaltic pump Column Preparation Procedure : 1. Pour 1L 0.5N NaOH into 5 x
Large Scale Tubulin Preparation
2. Collect homogenate (~8-9 liters), transfer into 36 GSA bottles and spin 90' at 12K in a GSA rotor at 4¡C. 3. Collect supernatant and transfer 1 liter to a 1.8L glass Fernbach flask that has 500 ml of 37¡C glycerol. Add 0.1 mM GTP, 0.5 mM ATP
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