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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312446310
中文名称:
英文名称:X12 TUBING,PVC,400MM LONG,BORE 1.02MM (PACK OF 12)
货期:热销品现货,其他请咨询




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文献和实验Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
mixture to cool to room temperature before finally correcting pH. Make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 15 min. Preparation of Red blood cell lysis buffer: 0.01 M Tris-HCl pH 7.6, 320 mM sucrose, 5 mM MgC12, 1% Triton X
Long PCR Reagents and Guidelines
targets (1). 1X Long PCR Buffer 85 mM KOAc 25 mM Tricine pH 8.7 (adjust pH of Tricine stock solution with KOH) 8% glycerol 1% DMSO (1 to 4% works) 1.1-1.3 mM Mg(OAc)2 A 5X buffer stock containing 6 mM Mg(OAc)2 (1.2 mM final concentration
Long-PCR Reagents and Guidelines
. For PCR with low-complexity templates (e.g., plasmid and cosmid inserts) 50 microliter reaction in 1X Long-PCR buffer (5X recipe below) 1-2.5 U Tth 0.02 U Vent 0.2 mM each dNTP 20-40 pmoles each primer (400-800 nM) 1.1-1.2 mM Mg(OAc)2
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