赛默飞世尔Thermo Fisher IV SET W/2

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2

Differential Display of RNAs

, T12 MG, T12 MT ; where M is A,C or G) dilute DNA-free RNA to 0.1 µg/µl with H2 ODEPC , keep on ice set up cDNA synthesis reaction for each degenerate anchored oligo(dT) primer set: 4 µl 5 x reverse transcriptase buffer, 2 µl DTT

PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)

sample DNA templates were amplified at different annealing temperatures as shown: note that while plasmid is amplified from 37 to 55℃, HPV DNA is only specifically amplified at 50℃.Primer Length The optimum length of a primer depends upon its （A+T