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货号:T_70104-355-92
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英文名称:SANIHOL 70 DENAT ETHANOL 1GAL
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
polymerase (REDTaq; Sigma D4309) (for polymerase chain reaction [PCR]; see Step 45 and Discussion) dNTP set (100 mM; Invitrogen) (for PCR; see Step 45 and Discussion) Ethanol (70% and 100%) Fetal bovine serum (10% in PBS) Formaldehyde
acids A. General rules Most nucleic acids may be precipitated by addition of monovalent cations and two to three volumes of cold 95% ethanol, followed by incubation at 0 to -70 degC. The DNA or RNA then may be pelleted by centrifugation at 10
acids may be precipitated by addition of monovalent cations and two to three volumes of cold 95% ethanol, followed by incubation at 0 to -70 ℃. The DNA or RNA then may be pelleted by centrifugation at 10 to 13,000 x g. for 15 minutes at 4℃. A subsequent
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