赛默飞世尔Thermo Fisher BRUTE 32 GA

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge

重组DNA的分离、克隆与测序实验

, extracted twice with equal volume ether, ethanol precipitated, and the DNA pellet is rinsed and dried.   F. Kinase end-labeling of DNA Typical 5'-kinase labeling reactions included the DNA to be labeled, [[gamma]]-32-P-rATP, T

重组DNA的分离、克隆与测序实验手册

, [[gamma]]-32-P-rATP, T4 polynucleotide kinase, and buffer (3). After incubation at 37℃, reactions are heat inactivated by incubation at 80℃. Portions of the reactions are mixed with gel loading dye and loaded into a well of a polyacrylamide gel