赛默飞世尔Thermo Fisher 1000 UG/G K

核酸电泳工作流程——5 大主要步骤

), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360

The ribonuclease protection assay (RPA)

from underneath the oil (try to avoid the oil) and transfer to the tubes containing the Proteinase K solution. Quick vortex, quick spin in the microfuge, and incubate for 15 minutes at 37°C. 17. Add 65 µl Tris-saturated phenol and 65 µl chloroform:isoamyl

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);