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- 文献和实验
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- 库存：
999
- 供应商：
北京泽平
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- 保修期：
1年
- 规格：
EA
货号：T_70310237811
中文名称：
英文名称：X6 N Needle (21/51/2)
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
until it becomes thoroughly wetted. Poke 3 widely spaced wholes in an asymmetrical pattern through the filters and the agar near the edge of the dish with a 18G or 21G needle for future orientation (see Figure 1). Place Saran wrap on benchtop. Spot out 0.5 mL puddles
Hints and precautions for the care, feeding and breeding of Neurospora
. 4:21-25). The formula in Davis and de Serres, p. 86, incorporates this change. The trace elements used for Vogel''s medium N are suitable for SC at 0.1 ml per liter final volume; there is no need for a separate formula. Russo, et al. (1985 N.N. 32
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