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999
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北京泽平
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货号：T_70115-177-815
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英文名称：ALKALINE-CYANIDE, LIQUID REAGE
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文献和实验In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
10 min in 50% HB / 50% PBTw at 48° 2 x 10 min in PBTw at 48° 1 x 5 min in PBTw at room temperature. 20) Incubate 15 min in PBTw / 0.5 mg/ml BSA (either fraction V or restriction enzyme grade). 22). Dilute alkaline
RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes
alkaline-phosphatase-conjugated anti-Dig (Fab2 fragment) from Roche (#1 093 274); the optimal dilution of this antibody has to be decided empirically. It varies from lot to lot. We have been using dilutions from as low as 1 : 1000 to as high as 1
., Elhajouji, A., Belpaeme, K., and Lison, D. 1997. In vitro genotoxic effects of hard metal particles assessed by alkaline single cell electrophoresis and elution assays. Carcinogenesis. 18:177‐184.
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