赛默飞世尔Thermo Fisher CHLORODIBRO

核酸电泳工作流程——5 大主要步骤

), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360

TISSUE CULTURE ON COVERSLIPS

, into a sterile 15ml conical centrifuge tube. V. Reagents: Chang media: IRVINE cat# T100-018, liquid and frozen supplement,100ml bottle, store supplement frozen, basal media at 4° C. Colcemid: GIBCO cat# 15210-040. 10µg/ml in Hank's

Counting ES cell Chromosomes

for four hours in the presence of colcemid (3.125 µl of 10 µg/ml colcemid (Gibco # 15212-012) per ml of medium). 2ml per 35 mm and 4ml per 60 mm. 3) Remove media and place it in a 15 ml Falcon tube. Wash with trypsin, and trypsinize with 1.5 ml trypsin.