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货号：T_70113-641-277
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英文名称：2123621T RINSE 25/PK
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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Transient transfection into 293T cells
. Less than 50% confluence may be OK but the amount of protein expressed will be low because of the small numbers of cells. 2. One hour prior to the transfection, change to medium containing 25µM chloroquine (from x1000 stock in PBS, stored at -20°C
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