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999
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北京泽平
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货号：T_70122-026-405
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英文名称：WATER HIGH PURITY 1-GAL 4/CS
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文献和实验Replication timing by density transfer
(until budded cells accumulate). Remove one sample (t = 0), then swirl the flask in ice-water and return it to 23o C. Continue to collect samples (until t = ~80 min). The ice-water treatment is to quick-chill the culture to 23o C, and is usually
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
to vortex mix the carrier DNA before pipetting it. 5. Incubate the tube in a water bath at 42°C for 40 to 60 min. Many laboratory strains will yield up to 1 x 105 transformants/mg plasmid after 60 min incubation. Extending the time at 42°C to 180 min
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