赛默飞世尔Thermo Fisher LB MEDIUM (

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

separately. Isopropyl-β-D-thiogalactopyranoside (IPTG; 200 mg/mL) LB agar LB-ampicillin agar plates Alternatively, LB-kanamycin agar plates can be used (see Step 48). LB (Luria-Bertani) liquid medium Add the appropriate

Preparing Antibiotics Stock Solution and Ampicillin Agar Plates

for stocks. Add 200 µl to 100 ml LB-Agar/LB-Medium (100 µg/ml). 200 µg/ml for Ampicillin works also fine. Preparation of Agar Plates  Agar Recipes: 10 g of NaCl 10 g of Tryptone 5 g of Yeast Extract 20 g of Agar

Preparation of Antibiotic Stock Solutions and Agar Plates

solidify at room temperature, then put to 4℃. Stability of Agar plates: Of course stability problem is the same for medium and plates! Always add antibiotics to LB-Medium freshly and short before use! It is often said, that Ampicillin plates are stable