赛默飞世尔Thermo Fisher AL23 TUBE (

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge

Procedures for In Vitro Production of Bovine Embryos

) from each well of the 4-well plate and place in the microcentrifuge tube. Up to 300 embryos can be loaded in one microcentrifuge tube.5. Repeat steps 3 and 4 until all plates have been processed.6. Remove cumulus cells from embryos/oocytes by vortexing (Figure 18) the tube

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

of 20 mM HEPES Buffer in 0.9% saline solution. In a separate conical tube, create a T solution by adding 17 μl of the thrombin stock, and 1.3 μl of 2 N Ca solution to 135 μl of DMEM. See Table 1.    2)      In a third conical tube, prepare