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- 详细信息
- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
1年
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EA
货号:T_701K953916-0000
中文名称:
英文名称:INLET FILTER SS
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文献和实验Southern Hybridization Protocols
(Polyvinyl pyrrolidone) (Fisher Bioreagents) 300mg BSA (A-9647 Sigma) Top up autoclaved H2O up to 30ml (do NOT autoclave solution: BSA will coagulate!) Filter with 0.2 m . Store at -20℃ Preparation of 50% dextran sulphate (10ml) 5g Dextran sulphate 10
Large-scale yeast transformation
. Resuspend yeast in the 90 mls LiAc/TE, put back in same flask (rinsed with sterile water). Shake at 30o C for 30 mins. Add 600 ul beta-mercaptoethanol. Shake at 30o C for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3 . Cells ready
Large-scale yeast transformation大规模转化酵母【Yale University】
with sterile water). Shake at 30℃ for 30 mins. Add 600 ul beta-mercaptoethanol. Shake at 30℃ for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3 . Cells ready to use. 1.We use selection because we are maintaining a plasmid. Presumably the main thing
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