赛默飞世尔Thermo Fisher 2-BROMOPROP

DNA Solutions （DNA分离）

xylene cyanole FF (Kodak T-1579) ddH2 O to 10 ml (store at -20deg.C). Alkaline lysis solution (NaOH/SDS) : 0.2 N NaOH, 1% SDS in ddwater. 20 ml of 1 N NaOH (or 0.8 gms) 10 ml of 10% SDS (or 1.0 gms) ddH2 O to 100 ml (make fresh) 15% Ammonium

寡核苷酸的PAGE和DEAE纯化

slice solution to a Fisher screening column which has been placed in a 15 ml conical tube. Centrifuge for 1-2 min in clinical centrifuge. This step removes large pieces of acrylamide.5. Add the equivalent of 0.3 ml packed DEAE sepharose FF resin

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

(100 units/ml and 100 μg/ml respectively) and 1.98 g of glucose to 250 ml 1x sterile phosphate buffered saline (PBS) and bring solution volume to 500 ml with additional sterile 1x PBS.    2)      Prepare stop media by adding 25 ml FBS and 5 ml