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999
- 供应商：
北京泽平
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- 保修期：
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货号：T_70311730129
中文名称：
英文名称：FLOWTUBE 7ML/MIN DIRECT AIR 65MM
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmids* This protocol is designed to allow the use of 96-well trays (8x12 microtiter tray format
Direct tissue explant method for derivation and study of extravillous trophoblas
the culture plates at 37°C for 10 -15 min and then cover the gel in 1 ml of SFM, and store in a sealed humid box at 4°C until required.Selection of tissue Using scissors, cut numerous terminal portions (~2-3 mm) of the villous tree for explant culture
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
buffer R1 with RNAse A (0.2 mg/ml) and RNAse T1 (100 units/ml). Let rest for 10 min. on ice to relax pellets before gentle vortexing or shaking. [1] 4. Add 300 ul of Qiagen buffer R2 (lysis buffer) per well. Seal wells with plastic sealing tape and mix
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