赛默飞世尔Thermo Fisher 0.5ML PCR T

RT-PCR and PCR

Reverse transcription (RT) Materials required RT-PCR kit (Gibco) Primers (300 nM each) PCR thermocycler Microfuge Microfuge 0.5 ml thin walled PCR tubes Thermocycler Ð Caution

定量PCR简介

以500 rpm的速度旋转，能保证样品间具有极高的温度均一性（±0.01ºC），从而大大提高了反应的稳定性。 可同时检测FAM、JOE、ROX和Cy5等4种荧光信号，适应于多个目标基因的复合检测。 Rotor Gene 3000型荧光定量 PCR仪 的主要参数： Excitation Source: 470

PCR Amplification of DNA

mineral oil (for thermocyclers without a heated lid 1. Combine the following for each reaction (on ice) in a 0.2 or 0.5 ml tube:  10X PCR buffer