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999
- 供应商：
北京泽平
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货号：T_70310121981
中文名称：
英文名称：SPECIMEN JAR 1250ML mm
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文献和实验电镜样品制备方法 Specimen preparation for electron microscopy
Specimen preparation for electron microscopy非常详细的电镜样品制备方法 E.M. PROCESSING SCHEDULE - EPOXY RESIN Fix tissue in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer at 4o C, for a minimum of 4 hours. Tissue should be cut
Preparation Of Ciliated Protozoa For Scanning Electron Microscopy
) Gently pellet cells from the culture medium and suspend in room temperature HNMK ( 50 mM HEPES, pH 6.9, 36 mM NaCl, 0.1 mM Mg acetate, 1 mM KCl) at a concentration of 106-108 cells/ml. Put in a shallow container and gently agitate for 10-30 min at room
for four hours in the presence of colcemid (3.125 µl of 10 µg/ml colcemid (Gibco # 15212-012) per ml of medium). 2ml per 35 mm and 4ml per 60 mm. 3) Remove media and place it in a 15 ml Falcon tube. Wash with trypsin, and trypsinize with 1.5 ml trypsin.
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