赛默飞世尔Thermo Fisher TRAY RADIAT

基本实验技术

name, CAS#, health hazard data, including first aid treatment, physical data, fire and explosion hazard data, reactivity data, spill or leak procedures, and any special precautions needed when handling this chemical. A file containing MSDS information

Stripping Western Blots

: Use 4X stacking buffer at 1:8, 10% SDS at 1:5, and b-ME at 70μl/10ml (e.g.10mls strip=1.25mls 4X, 2mls 10% SDS, 70μl b-ME & 6.68mls H2O) Lysis of Cultures Cells General Protocol 1) Wash cells twice with ice-cold PBS. Use 3mls (6cm plate

Marcantonio Lab Protocol Manual

of TE buffer. Transfer to a 15 ml conical tube. Add 4.4 g of CsCl (Optical grade), dissolve by shaking, and add 0.4 ml of ethidium bromide (10 mg/ml). Caution: Ethidium bromide is a potent mutagen and an environmental hazard. Always wear gloves