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999
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货号：T_70113-700-415
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英文名称：GLS 80 CAP CS 20
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文献和实验Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
Northern Blotting方法(Howell Lab)
Fixing Bake membrane in 80℃ vacuum oven for 20 min. UV crosslink (on optimal setting of Fisher Crosslinker) the membrane (face side up). Store between 2 pieces of chromatography paper in a plastic bag. Labelling the probe
into several small screw-cap jars (~50 ml capacity) Store at room temperature 20 x Energy Regeneration System* 150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4 ) 40ml 0.5 M EGTA (2 mM EGTA)
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