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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310419742
中文名称:
英文名称:Comb, 1.5mm, 10 samples
货期:热销品现货,其他请咨询




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文献和实验做了两个月的时间的 western blotting 终于出了比较好的结果。 老板让整理出protocol 这是写的PROTOCOL 大概会有很多错误请大家指正!谢谢先 WESTERN PROTOCOL A. Preparation of Samples 1. Get the heads of the fly 2. Stir them in the lysis buffer(4heads/10ul) 3. Add 2XSDS-gel-loading buffer
cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick) Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel: Add 678 μl
Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
as follows:MDE gel 16.0mlddH2O 44.2ml10X TBE 3.84ml10% APS 256µlTEMED 25.6µlPour sequencing gel format with appropriate sharkstooth comb. Gel will polymerize in about 1 hour.Loading Buffer95% formamide10mM NaOH0.025% Bromophenol Blue0.025% Xylene CyanolRun
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