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英文名称:7,5M CLEAR C-FLEX TUBG 6,4X9,6MM
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文献和实验preparation of genomic DNA from C. elegans (or related nematodes). If you want a small scale prep, take a look at the original protocol or scale this one down.Solutions1) Worm lysis buffer; 0.1M Tris-Cl pH 8.5, 0.1M NaCl, 50 mM EDTA pH 8.0, 1% SDS. Store at RT
Preparation of Lactobacillus Competent Cells
2 ) 50mL Electroporation Buffer (0.5M Sucrose, 10%(v/v) glycerol) Cap the flasks with foil and autoclave. Once the MRS has cooled, inoculate one of the flasks with L.plantarum culture and grow overnight at 30°C. Day
is adjusted to 1.4M with 5M NaCl, 1/10 vol. of 10% CTAB added and samples incubated a further 10 min. at 65C. 4. Add 1 vol. chloroform:isoamyl alcohol, gently emulsify by inversion, incubate at 0C for 30 min. Spin 10 min. at 4C at rpm max. Transfer
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