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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310671692
中文名称:
英文名称:Comb, 1.5mm, 16 samples
货期:热销品现货,其他请咨询




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文献和实验Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
as follows:MDE gel 16.0mlddH2O 44.2ml10X TBE 3.84ml10% APS 256µlTEMED 25.6µlPour sequencing gel format with appropriate sharkstooth comb. Gel will polymerize in about 1 hour.Loading Buffer95% formamide10mM NaOH0.025% Bromophenol Blue0.025% Xylene CyanolRun
. The water should form an even sheet over the plate. Rinse thoroughly with tap distilled water, then double distilled water. Rinse with 95% Ethanol and wipe dry with kimwipes, make sure all dust is gone. Also wash spacers and comb with soap and water
Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
*dNTPS mix (final concentration): dATP 2.5mM; dTTP 2.5mM; dGTP 2.5mM; dCTP 1.25mM SSCP Gels Prepare 0.5x MDE gel as follows: MDE gel 16.0ml ddH2 O 44.2ml 10X TBE
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